Agarose Gel Electrophoresis is a standard method used to purify and separate fragments of DNA. DNA fragments of different sizes are separated from each other due to different migrational velocities during electrophoresis (in agarose gel) under the influence of an electric field. The presentation in this video tape starts with the scientific illustrations of the basic technique of electrophoresis and restriction enzymes. Scientific graphics/animations have been used to explain the basic principle of electrophoresis. Total length of video tape is 17 minutes.

Live footage covers following steps:

Preparation of gel/running buffer
Casting gel tray with agarose
Loading DNA samples
Running electrophoresis
Gel staining and DNA bands visualization.


Volume 1:

Introduction
Restriction & Modifying Enzymes
Bacteriophage Infection
Genomic Library
Plasmid Preparation
Gel Electrophoresis
DNA Subcloning
DNA Hybridization

Volume 2 :

Southern Blot & its applications
Dot Blot
DNA Sequencing
DNA Mutations
PCR (Polymerase Chain Reaction)
DNA Footprinting
Nuclease S1 Protection Assay
Polyacrylamide Gel Electrophoresis
Western Blot