Help?     MAPPING OF DNA BY RESTRICTION ENZYMES  Price Information Enough for 10 gels Load 4 different samples in a single gel Standard DNA is also provided Each gel can be shared by 2-3 students Understand DNA mapping by using restriction      enzyme digested (pre- digested) DNA samples. Understand principle of agarose gel electrophoresis. Understand the principles of DNA fingerprinting. Kit includes predigested and ready to load DNA      samples. Understand how DNA samples separate-based upon      different sizes. Learn how to stain, visualize and analyze DNA      samples. Understand the concept of mapping of genes. DNA mapping is a common technique used to determine the location of genes. Mapping of DNA by restriction enzymes not only provides the genetic information of DNA but it also provides the information about enzymatic cleavage sites which are used for subcloning DNA fragments into vectors. Subcloned DNA fragments are then used for DNA sequencing, protein expression and other molecular biology experiments. This experiment includes 8 different predigested DNA samples along with a standard DNA ladder. DNA fragments from each sample are separated on an agarose gel during electrophoresis. Depending upon the restriction enzymes used in each sample, a unique DNA pattern is obtained in a stained gel which is used to map the relative positions of restriction sites of the original DNA molecule. No human DNA is used in this experiment. Time Needed to Complete the Experiment: Casting the gel: 25 minutes. Loading DNA samples: 10 minutes. Running time for electrophoresis: 45 minutes. Staining time: 2-3 hrs for methylene blue stain or instant results with ethidium bromide stain. Kit includes following items: Predigested DNA samples (pre-cut with different restriction enzymes). 5 different samples (each 100 µl); Each sample contains different sizes of DNA      fragments. Standard DNA (100 µl) for comparing fragments of various sizes. Loading pipet of fix 20 µl volume. This pipet is extremely durable which can be used up to      5,000 times. Microfuge tubes rack of 96 loading tips. Heavy duty test tube rack containing 80 holes for microfuge tubes. Agarose, enough for 10 gels Concentrated electrophoresis buffer, enough for 10 gels DNA staining solution. Gel running buffer. Measuring tubes (15 ml and 50 ml capacity) & microfuge tubes. Instructions sheet Blank sample for agarose gel as practice. Videotape of Agarose Gel Electrophoresis. Other Requirements: Beakers, Hot Plate or Microwave. Catalog# Description Price Discounted Price         204-1 1 kit of Gel Electrophoresis (Size determination) $89.99 $69.99 204-2 3 kits of Gel Electrophoresis (Size determination) $259.99 $194.99 ($65 each) 204-3 5 kits of Gel Electrophoresis (Size determination) $419.99 $309.99 ($62 each) 204-4 6 sets of Refill DNA (each set has 7 samples & standard DNA) $129 $99.99 ($16.65 each) 204-5 Extra agarose, buffer and staining solution (for 10 gels) $30 $24.99 Shipping & Handling: 7% of total order. Minimum S & H: $8 (USA), and $11 (Canada).           Back to Main >>
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