Enough for 12 gels. Restriction enzymes are included
Each gel can be shared by 2-3 students
Learn how restriction enzymes are used as most powerful
tools in biotechnology.
Learn how various enzymes cut DNA at unique recognition
sequences.
Visualize and analyze DNA samples by running agarose
gel electrophoresis.
Understand how DNA samples separate on a gel, based
upon different sizes.
Learn how to stain, visualize DNA samples following
agarose gel electrophoresis.
In this experiment different restriction enzymes are used
to digest linear lambda DNA at unique nucleotide sequences.
Bacteriophage lambda DNA has a linear structure with 48,
502 base pairs which gives a unique pattern of DNA fragments
on agarose gel after cutting with restriction enzymes..
Different restriction enzymes recognize specific sequences
of this large molecule of DNA. Following Agarose gel electrophoresis,
staining of gel will yield distinct DNA bands depending
upon the type of the enzyme used for cutting the DNA.
Time Needed to Complete
the Experiment:
Digestion of DNA by restriction enzymes: about 1 hour.
DNA can also be digested overnight
with enzymes. Cut DNA can be saved in freezer for future
use.
Casting the gel: 25 minutes.
Loading DNA samples: 10 minutes.
Running time for electrophoresis: 45 minutes.
Staining time:
2-3 hrs for methylene blue stain or instant results with
ethidium bromide stain.
Kit
includes following items:
Uncut Lambda DNA
Set of restriction enzymes.
Buffer for enzymes
Double autoclaved sterile water
Standard DNA for comparing DNA fragments.
Loading pipet of fix 20 µl volume. This pipet is
extremely durable which can be used up to 5,000
times.
Microfuge tubes rack of 96 loading tips.
Heavy duty test tube rack containing 80 holes for microfuge
tubes
Agarose, enough for 10 gels
Concentrated electrophoresis buffer, enough for 10 gels
DNA staining solution and gel buffer
Measuring tubes (15 ml and 50 ml capacity) & microfuge
tubes.
Instructions sheet.
Videotape of Agarose Gel Electrophoresis.
Gel loading dye.
Blank sample for agarose gel as practice.
Other Requirements:
Microwave, Incubator (optional), Glassware, Hot Gloves
Catalog#
Description
Price(1
Kit)
Price(3
Kits)
Price(5 Kits)
311-1
Restriction digestion of lambda DNA.
30 preps
$59
$167
$259
312-1
Extended Genomic DNA preps*
$84
$239
$389
*Extended
kit includes extra reagents (for visualization of
DNA during electrophoresis) such as loading pipet,
agarose, microfuge tube rack, gel buffer, standard
DNA, staining solution and agarose electrophoresis
videotape etc. Note: Due to restriction enzymes,
cold shipment charges will apply.
Enough for 12 gels. Restriction enzymes are included 
Learn how restriction enzymes are used as most powerful
tools in biotechnology.
Digestion of DNA by restriction enzymes: about 1 hour.
DNA can also be digested overnight
with enzymes. Cut DNA can be saved in freezer for future
use.
