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BLUE/ WHITE SCREENING OF COLONIES (30 Preps)
 Price Information
Enough for 30 preparations
Load isolated samples (along with standard DNA) in a single gel
Each gel can be shared by 2-3 students

Simple and easy method to visualize ß-galactosidase      expression.
Understand identifiable DNA traits which give blue or      white colonies.
Learn how to streak E.coli cells on bacterial plates.
Understand how bacteria propagate in presence of      antibiotic.

   

Plasmid DNA such as pUC vector is very common vector which produces ß-galactosidase after hydrolyzes of X-Gal. When a foreign DNA fragment is subcloned into the pUC polylinker region, gene for the ß-galactosidase interrupted, resulting in an inability to hydrolyze X-Gal. In presence of X-Gal and IPTG (inducer), such interruption of ß-galactosidase gene results in the production of white colonies. In this experiment both white and blue colony forming plasmids are provided. Pre-transformed E.coli cells are provided (no dry ice necessary for shipping) which are stable for long time at room temperature. E.coli cells containing plasmid are grown on bacterial plates containing Ampicillin. The new traits of DNA are identifiable as blue or white colony after the transformation process.

Time Needed to Complete the Experiment:
Preparation of antibiotic resistant plates: About 40 minutes. Plates containing agar media      can be stored in refrigerator for up to three months.
Inoculation of plates with pre-transformed plasmid DNA: 5 minutes.

Results:
After 16-24 hours of incubation of plates at 37 °C or about two days of incubation at room temperature.

Kit includes following items:
Two sets of pre-transformed plasmid DNA in E. coli cells (Blue and white colony making      plasmids).
LB Media for bacterial growth.
Ampicillin-antibiotic for bacterial plates.
LB Agar Media for making bacterial plates.
Petri plates for pouring agar media.
X-Gal/IPTG Solution.
Microfuge tubes.
Gel running buffer.
Sterile inoculating loops.
Instructions sheet.


Other Requirements:
Microwave, Incubator (optional), Glassware, Hot Gloves


Catalog#
Description
Price(1 Kit)
Price(3 Kits)
Price(5 Kits)
         
309-1 White blue Screening of colonies (for 30 plates)* $59 $167 $259
*Kit includes 30 petri plates and growth media for making culture plates.
 
 
 
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